Gradient Elution of Disaccharides on a Stearic Acid-Treated Charcoal Column

Published in: 1958
Source: Analytical Chemistry
Vol. 30 (7): 1294-1296 (1958)

 

Excerpt:

During investigations on the minor sugars of honey, a technique for the isolation of disaccharides, not separable by paper chromatography, was needed. This paper describes the separation by gradient elution of four such pairs of sugars: turanose-sucrose, isomaltose-gentiobiose, maltulose-nigerose, and melibiose-lactose. Four other pairs of sugars (maltulose- sucrose, maltulose-maltose, sucrose-
maltose, and turanose-isomaltose), although separable on paper, were also separated by this method.

 

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