Excerpt:
WHEN cryptostegia leaves (C,. grandiflora) were subjected to anaerobic fermentation by Clostridium roseum as a pre-treatment in recovery of rubber, the cell walls were digested and the cell contents (protoplasts) liberated. The protoplasts remained as discrete entities and could readily be separated from the bagasse (cuticle, ribs, vascular tissue). Since the fractionation
of the leaves was so clean cut and the recovery of the protoplasts and of their water-insoluble constituents so high, it was deemed advisable to apply the fermentation process to other plant materials as a first step in the preparation of proteins and lipids.
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