Excerpt:
Methods recommended (1) for the carbohydrate analysis of honey are little changed from those used 50 years ago. They are highly empirical and based upon insufficient knowledge of the sugars present in honey. In a recent study (2) of the determination of glucose and fructose, the authors examined five procedures and concluded that variance due to methods was as great as that due to differences among various floral types of honey. Thus, little confidence can be placed in comparison of results of analyses of honey by different existing methods. Taufel and Reiss (3) have concluded from a paper-chromatographic study of honey that the customary methods of analysis are inadequate.
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